Fig. 5.

Contactin1 Immunohistochemistry (downregulated on gene array at P90). (A) Retinal cross sections from Pde6b^rd1/rd1 and Pde6b^wt/wt stained for DAPI (blue), CHX10 (green) and Contactin 1 (red). ONL – Outer nuclear layer, OPL – Outer Plexiform layer, INL- Inner nuclear layer, IPL – inner plexiform layer, GCL – Ganglion cell layer. Scale bar = 20 μm. White box indicates area from which close up images in panels B & D are taken. (B&D) Close up images of bipolar cell bodies in Pde6b^rd1/rd1 retina (B) and Pde6b^wt/wt (D) at four time points during degeneration (P40, P90, P120, P150). CHX10 (green) and Contactin 1 (red). Scale bars = 5 μm. (C) Colocalised pixels above threshold - a semiquantitative index of protein staining. Normalised to highest value over all retinae stained for Contactin 1. Dots represent the mean value for each animal, lines connect the means for each group at that timepoint. Star markers on graph represent adjusted p values, Sidak's method for multiple comparison at P90 p = 0.0026) (see results section for details). red line = Pde6b^rd1/rd1; blue line = Pde6b^wt/wt. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)