Table 2.
The 20 most abundant differentially accumulated proteins (DAPs) in the Ay-integrated lines of each of three wheat cultivars
| First accession | Protein name | iBAQ score | Proportion (%) | Line 1 |
Line 2 |
||
|---|---|---|---|---|---|---|---|
| FC | p-value | FC | p-value | ||||
| cv. EGA Gregory | |||||||
| TraesCS5D01G368900.1 | Serpin family protein | 1,511,675 | 5.9 | 1.08 | 0.684 | 1.27 | 0.036 |
| TraesCS1A01G066100.1 | 11S globulin seed storage protein | 979,705 | 3.82 | 0.98 | 0.974 | 1.34 | 0.025 |
| TraesCS5D01G425800.1 | Serpin family protein | 447442.5 | 1.75 | 1.22 | 0.247 | 1.7 | 0.001 |
| TraesCS3B01G111200.1 | Dimeric alpha-amylase inhibitor | 199942.5 | 0.78 | 1.42 | 0.029 | 1.46 | 0.018 |
| TraesCS6B01G383500.1 | Dehydrin | 178,020 | 0.69 | 1.54 | 0.063 | 1.62 | 0.033 |
| TraesCS5B01G012300.1 | Enolase | 147929.3 | 0.58 | 1.2 | 0.091 | 1.24 | 0.046 |
| TraesCS5A01G469300.1 | 40S ribosomal protein S26 | 140878.3 | 0.55 | 1.17 | 0.067 | 1.27 | 0.007 |
| TraesCS1D01G067100.1 | 11S globulin seed storage protein | 138,015 | 0.54 | 0.97 | 0.948 | 1.39 | 0.014 |
| TraesCS3A01G305400.1 | Aspartate aminotransferase | 108183.8 | 0.42 | 1.14 | 0.102 | 1.24 | 0.008 |
| TraesCS5D01G395600.1 | Late embryogenesis abundant D-like protein | 96,284 | 0.38 | 1.34 | 0.037 | 1.38 | 0.022 |
| TraesCS1B01G144300.1 | 50S ribosomal protein L5 | 93947.25 | 0.37 | 1.3 | 0.01 | 1.14 | 0.216 |
| TraesCS5D01G479200.1 | Wound-induced protease inhibitor | 62676.5 | 0.24 | 0.61 | NA | Absent | NA |
| TraesCS1A01G372700.1 | Late embryogenesis abundant protein | 54,874 | 0.21 | 1.2 | 0.099 | 1.27 | 0.028 |
| TraesCS7D01G313800.1 | 60S acidic ribosomal protein P1 | 48198.5 | 0.19 | Absent | NA | 0.89 | NA |
| TraesCS6B01G424800.1 | 50S ribosomal protein L14 | 42962.25 | 0.17 | 0.75 | 0.016 | 0.72 | 0.009 |
| TraesCS7D01G239400.1 | Caffeoyl-CoA O-methyltransferase | 36,792 | 0.14 | Absent | NA | 0.94 | NA |
| TraesCS7B01G067000.1 | Aldose 1-epimerase | 36,131 | 0.14 | 0.65 | 0.011 | 0.84 | 0.248 |
| TraesCS5B01G179800.1 | Phosphoenolpyruvate carboxylase | 34,152 | 0.13 | 0.81 | 0.04 | 0.96 | 0.765 |
| TraesCS2D01G206300.1 | Aquaporin | 33047.5 | 0.13 | 1.06 | 0.61 | 1.3 | 0.003 |
| TraesCS5D01G454800.1 | Thioredoxin | 23,402 | 0.09 | Absent | NA | 1.2 | NA |
| cv. Bonnie Rock | |||||||
| TraesCS4B01G328100.1 | Dimeric alpha-amylase inhibitor | 2,569,800 | 5.97 | 1.21 | 0.019 | 0.98 | 0.926 |
| TraesCS1D01G067100.1 | 11S globulin seed storage protein | 1,155,700 | 2.68 | 1.35 | 0.002 | 1.37 | 0.002 |
| TraesCS1B01G330000.1 | High-molecular-weight glutenin subunit | 517,415 | 1.2 | 1.58 | 0 | 1.51 | 0.001 |
| TraesCS4B01G262700.1 | Vicilin-like antimicrobial peptides 2-2 | 371162.5 | 0.86 | 0.82 | 0.024 | 0.8 | 0.021 |
| TraesCS7D01G133500.1 | Alkaline alpha-galactosidase seed imbibition protein | 330,510 | 0.77 | 1.17 | NA | Absent | NA |
| TraesCS4A01G214200.1 | Protein disulfide isomerase | 329817.5 | 0.77 | 1.22 | 0 | 1.29 | 0 |
| TraesCS5D01G425800.1 | Serpin family protein | 223,240 | 0.52 | 1.23 | 0.012 | 1.18 | 0.062 |
| TraesCS2B01G381600.1 | Glutaredoxin | 152,480 | 0.35 | 0.82 | 0.008 | 0.78 | 0.004 |
| TraesCS4D01G046400.2 | 14-3-3 protein | 129182.5 | 0.3 | 0.83 | 0.019 | 0.85 | 0.055 |
| TraesCS7D01G335000.1 | 60S ribosomal protein L22, putative | 105966.3 | 0.25 | 1.21 | 0.114 | 1.33 | 0.027 |
| TraesCS1D01G266100.1 | Wound-induced protease inhibitor | 85674.67 | 0.2 | 0.81 | 0.206 | 0.57 | 0.012 |
| TraesCS4D01G109200.1 | Ribosomal protein L3 | 77344.25 | 0.18 | 1.38 | 0 | 1.28 | 0.003 |
| TraesCS5A01G385600.1 | Late embryogenesis abundant D-like protein | 70,333 | 0.16 | 1.1 | 0.195 | 0.79 | 0.01 |
| TraesCS6A01G093200.1 | Endoglucanase | 68401.25 | 0.16 | 1.21 | 0.048 | 1.2 | 0.085 |
| TraesCS6A01G049400.1 | Alpha-gliadin | 50,441 | 0.12 | Absent | NA | 1.31 | NA |
| TraesCS2B01G004800.1 | Alpha-amylase inhibitor protein | 39,659 | 0.09 | Absent | NA | 0.51 | NA |
| TraesCS5D01G150000.2 | ADP-ribosylation factor | 35927.25 | 0.08 | 1.12 | 0.494 | 1.33 | 0.04 |
| TraesCS5B01G478200.1 | Wound-induced protease inhibitor | 32010.5 | 0.07 | 0.74 | 0.235 | 0.54 | 0.04 |
| TraesCS7D01G340400.1 | Late embryogenesis abundant D-like protein | 31,912 | 0.07 | 0.96 | 0.534 | 0.73 | 0.001 |
| TraesCS1D01G144000.2 | 60 kDa chaperonin | 31585.75 | 0.07 | 1.3 | 0.01 | 1.26 | 0.029 |
| cv. Yitpi | |||||||
| TraesCS5D01G368900.1 | Serpin family protein | 1,485,900 | 2.05 | 1.08 | 0.467 | 1.29 | 0.004 |
| TraesCS5B01G434100.1 | Globulin-1 | 1,450,825 | 2 | 1.1 | 0.517 | 1.4 | 0.003 |
| TraesCS3A01G033900.1 | Heat shock protein | 1,261,755 | 1.74 | 1.55 | 0 | 1.26 | 0.021 |
| TraesCS5B01G488800.1 | rRNA N-glycosidase | 1,144,263 | 1.58 | 1.02 | 0.965 | 1.3 | 0.022 |
| TraesCS1D01G317300.1 | High-molecular-weight glutenin subunit | 1,069,063 | 1.47 | 1.11 | 0.628 | 1.55 | 0.002 |
| TraesCS7D01G066400.1 | Peroxidase | 539,750 | 0.74 | 1.05 | 0.798 | 1.26 | 0.015 |
| TraesCS5A01G417800.1 | Serpin family protein | 392782.5 | 0.54 | 1.35 | 0.073 | 1.53 | 0.009 |
| TraesCS1B01G034400.1 | Chymotrypsin inhibitor | 278293.3 | 0.38 | 0.63 | 0.03 | 0.63 | 0.029 |
| TraesCS1B01G081000.1 | Protease inhibitor/seed storage/lipid transfer protein family protein | 195,055 | 0.27 | 0.93 | 0.55 | 0.75 | 0.012 |
| TraesCS2D01G261500.1 | Eukaryotic translation initiation factor 5A | 109,729 | 0.15 | 1.31 | 0.033 | 1.18 | 0.217 |
| TraesCS2B01G047400.1 | Heat shock protein 90 | 109451.8 | 0.15 | 1.25 | 0.01 | 1.03 | 0.868 |
| TraesCS6D01G049100.1 | Heat shock 70-kDa protein | 80967.75 | 0.11 | 0.8 | 0.002 | 0.85 | 0.014 |
| TraesCS2D01G128900.1 | Cysteine proteinase inhibitor | 72805.75 | 0.1 | 0.59 | 0.003 | 0.96 | 0.899 |
| TraesCS2D01G348800.1 | Chitinase | 35795.5 | 0.05 | 0.57 | 0.016 | 1.14 | 0.522 |
| TraesCS7D01G411700.1 | Defensin | 32,103 | 0.04 | 0.4 | NA | Absent | NA |
| TraesCS7B01G184100.1 | HSP20-like chaperones superfamily protein isoform 3 | 31421.5 | 0.04 | 0.87 | 0.315 | 1.27 | 0.043 |
| TraesCS1D01G424500.1 | Programmed cell death protein 4 | 29,581 | 0.04 | 1.25 | 0.036 | 1.17 | 0.105 |
| TraesCS5B01G003500.1 | Grain softness protein | 25,665 | 0.04 | Absent | NA | 0.32 | NA |
| TraesCS1D01G306800.1 | 60S ribosomal protein L30 | 25,076 | 0.03 | Absent | NA | 0.86 | NA |
| TraesCS6A01G371100.1 | Aldehyde dehydrogenase | 24480.5 | 0.03 | 1.17 | 0.006 | 0.96 | 0.611 |
The proportion column contains the percentage of iBAQ score of each protein to the summed protein iBAQ score. The fold change (FC) and t test p-value were collected from supplemental Table S4.