Figure 2.

Transcript level of Perm1, PGC-1s, and ERRs increased during cardiac development and maturation. Protein and RNA were extracted from cardiac tissues at a different time points of embryogenesis, from neonatal pups and adult mice. mRNA levels for: (A) Perm1, (B) PGC-1α, (C) PGC-1β, (D) ERRα, (E) ERRγ, and (F) ERRβ were determined by RT-qPCR, normalized by 36B4 levels, and expressed relative to levels of each gene in P0 heart, which was set = 1. Data are the mean ± SD, expressed relative to P0 (n = 4). ∗p < 0.05 versus P0. (Whole heart tubes were sampled at E9.5, with ventricular tissue used at later stages.)