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. 2021 May 23;297(1):100825. doi: 10.1016/j.jbc.2021.100825

Figure 5.

Figure 5

Perm1 selectively regulates genes essential for oxidative metabolism. Primary cultured NCM were infected with adenoviruses expressing LacZ or Perm1. Forty-eight hours after infection later, the cells were harvested, followed by RNA extraction. A–D, mRNA levels for indicated genes were determined by qRT-PCR, normalized by 36B4 levels, and expressed relative to levels of each gene in LacZ infected cells = 1. Data are the mean ± SD. ∗p < 0.05 versus LacZ. Data are the mean of six experimental replicates from two representative experiments. E and F, whole cell protein lysates were subjected to western blot using antibodies as indicated (E). Sirt3 and Ckmt2 protein levels were quantified relative to expression level of LacZ (control) infected cells = 1 (F). Data are the mean of six experimental replicates from two representative experiments. ∗p < 0.05 versus LacZ.