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. 2021 May 23;297(1):100825. doi: 10.1016/j.jbc.2021.100825

Figure 6.

Figure 6

Perm1 binds PGC-1α and increases ERR-dependent transcriptional activity. A, U2OS cells were transfected with the pERRE-LUC reporter (40 ng) and pcDNA3 (control) or ERR expression plasmids (10 ng), together with pcDNA3 control (−), PGC-1α (5 ng) (+), in the presence of pcDNA3 control (white bars), Perm1 (10 ng) (gray bars), or Perm1 (40 ng) (black bars), plasmids. ∗p < 0.05. Data are the mean ± SD, of eight experimental replicates from two representative experiments. B, interaction of Perm1 and PGC-1α in vitro. HEK293 cells were transfected with pcDNA3-PGC-1α in combination with control or FLAG-Perm1 plasmid (A Flag-tag with a Kozak sequence was inserted into the N-terminus of full-length Perm1). Protein complexes were immunoprecipitated with an anti-FLAG antibody and then immunoblotted with an anti-PGC-1α antibody or anti-Perm1 antibody. C, interaction of Perm1 and PGC-1α in vivo. Whole ventricular tissue protein lysates from adult C57Bl6 mice were subjected to immunoprecipitation with anti-PERM1 antibody and then then immunoblotted with an anti-PGC-1α antibody or anti-Perm1 antibody. Representative blots are from two independent experiments. IP, immunoprecipitation.