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. 2021 May 23;297(1):100825. doi: 10.1016/j.jbc.2021.100825

Figure 7.

Figure 7

Perm1 enhances recruitment of PGC-1α to target gene promoter. A, C2C12 myotubes were infected with LacZ (white bars), PGC-1α (Flag-tagged) (black bars), Perm1 (striped bars), or both PGC-1α (Flag-tagged) and Perm1 (gray bars). ChIP assays were performed using anti-FLAG antibody, anti-PERM1 antibodies, or IgG (control). B, ChIPs were performed with adult mouse ventricular tissues using anti-PERM1, anti-ERRα, or anti-PGC-1α antibodies. The abundance of the Sirt3 ERRE, the Ckmt2 ERRE, and a negative control genomic region in the ChIPs were quantified by qPCR, normalized to the input signal, and expressed relative to the levels of each region in the control IgG sample. In A and B, data are the mean ± SD. A, ∗p < 0.05 versus LacZ. #p < 0.05 versus FLAG-PGC-1α. The data are the mean of eight experimental replicates from two representative experiments. B, ∗p < 0.05 versus IgG. The data are the mean of six different heart samples.