Table 3.
Cytometer specifications
Laser (wavelength) | Fluorochrome (marker) | BP filter | LP filter | Detection range | PMT voltage used |
---|---|---|---|---|---|
Blue (488 nm) | SSC | 488/10 | 345 | ||
Blue (488 nm) | FSC | 273 | |||
Blue (488 nm) | PE-Texas red (PI) | 610/20 | 595LP | 600–620 nm | 535 |
Blue (488 nm) | FITC (GFP) | 530/30 | 505LP | 515–545 nm | 480 |
Red (633 nm) | Alexa Fluor 647 (anti-spike antibody staining) | 660/20 | 650–670 nm | 550 |