Figure 6.

Coimmunostaining for HSV-1 and Aβ aggregates using anti-gB in aged 5XFAD mice. Coimmunostaining for Aβ aggregates (H31L21 antibody, green), HSV-1 virus (anti-gB antibody, red), and nuclei (DAPI, blue) in 7- to 10-month-old 5XFAD mice, which were challenged IC with 5 × 10³ PFUs of McKrae and succumbed to acute herpes simplex encephalitis within 96 to 200 h postinoculation (n = 8 mice) (A) and uninoculated age-matched 5XFAD (n = 5 mice) (B). Animals #1 and #2 in McKrae-challenged group (A) showed strong fluorescence signal intensity and elaborate aggregates morphology in the anti-gB channel, whereas animals #3 had the same signal intensity in the anti-gB channel as control group. Arrows point at staining of blood vessels. Scale bars = 20 μm.