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. 2021 May 24;297(1):100826. doi: 10.1016/j.jbc.2021.100826

Table 1.

Biological characterization of WT mAb1 and the mutants

Mutation aNormalized G0-F ADCC potency FcγRIIIa-binding affinity, KD (nM) Antigen-binding affinity, KD (nM)
WT 6.52% 100.0 ± 6.7% 266 ± 3 113
HC: N201A,N203A,K210A 6.43% 111.5 ± 3.5% 274 ± 8 119
HC: N201G,N203D,K210A 6.41% 120.5 ± 0.5% 273 ± 8 125
a

Normalized G0-F = [G0-F ÷ (G0-F + G0)] × 100%; detailed glycosylation profile is shown in Table S2.