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. 2021 May 26;297(1):100828. doi: 10.1016/j.jbc.2021.100828

Figure 2.

Figure 2

LL-34-I24A and LL-34-L31A peptides loose vetting activity in vivo. C57BL/6 mice ears were injected intradermally with either 25 μg of U1 dsRNA, 16 μM LL-34-parent, LL-34-I24A, and LL-34-L31A as shown in the figure for 24 h (n = 6). A, H&E staining of cross sections of mice injected with U1 dsRNA or LL-34 peptides is shown. The bar represents 20 μM. B–D, gene expression changes for IL-6, CXCL10, and TSLP were assessed with qRT-PCR. GAPDH was used as a reference gene. E–G, flow cytometry analysis was performed to assess the infiltration of immune cells in mouse ears injected with U1 dsRNA alone or a combination of U1 dsRNA and LL-34 peptides. Data are represented as the frequency of gated live cells for mast cells, macrophages, and neutrophils. (n = 3) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001). One way-ANOVA was used. LL-34, 34-amino acid peptide; ns, not significant.