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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Biomaterials. 2021 Apr 12;273:120797. doi: 10.1016/j.biomaterials.2021.120797

Figure 3. Short term IL-2 secretion modulated by substrate stiffness and ligand density.

Figure 3.

(A) IL-2 secretion of T cells characterized by flow cytometry. (B) IL-2 secretion of T cells activated for 4 hours on hydrogels with standardized coating (n=12 across 5 independent experiments), 0.1x coating (n=3 across 2 independent experiments), and 10x coating. Data are mean ± s.d., n=6 across 2 independent experiments. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, 2-way ANOVA, Tukey multiple comparison test. (C) IL-2 secretion of CD4+ and CD8+ T cell subsets. Data are mean ± s.d., n=12 across 5 independent experiments. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, 2-way ANOVA, Tukey multiple comparison test.