(A) IL-2 secretion of T cells activated for 4 hours on hydrogels in presence of Blebbistatin compared to DMSO controls. Data are mean ± s.d., n=7 across 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001, 2-way ANOVA, Tukey multiple comparison test. (B) IL-2 secretion of T cells activated for 4 hours on hydrogels in presence of Labtrunculin B compared to DMSO controls. Data are mean ± s.d., n=6 across 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001, 2-way ANOVA, Tukey multiple comparison test.