Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Biotinylated Goat-anti-mouse | BioLegend | 405303 |
| Mouse-anti-human CD3 (OKT3) | BioXCell | BE0001-2 |
| Mouse-anti-human CD28 (9.3) | BioXCell | BE0248 |
| Biological Samples | ||
| Leukopack | New York Blood Center | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Acrylamide (40%) | Sigma | 01697 |
| Bis-acrylamide (2%) | Fisher | BP1404-250 |
| Ammonium persulfate (APS) | Sigma | A3678 |
| Tetramethylethylenediamine (TEMED) | Sigma | T7024 |
| Streptavidin-acrylamide | Fisher | S21379 |
| (3-Aminopropyl)triethoxysilane (APTES) | Sigma | 440140 |
| Glutaraldehyde | Sigma | G7776 |
| Alexa-fluor 488 | Thermo | A20000 |
| Blebbistatin | Sigma | 203391 |
| Prolong Diamond | ThermoFisher | P36961 |
| Qiazol | Qiagen | 79306 |
| Critical Commercial Assays | ||
| Human IL-2 Secretion kit (APC) | Miltenyi Biotec | 130-090-763 |
| RosetteSep Human T cell Enrichment Kit | Stemcell Technologies | 15061 |
| CellTrace CFSE Dilution Assay | ThermoFisher | C34554 |
| CellVue Jade | ThermoFisher | 88-0876-16 |
| Software and Algorithms | ||
| FlowJo 7.6 | FlowJo LLC | |
| FCS Express V6 | De Novo Software | |
| Graphpad Prism 7 | Graphpad Software | |
| kallisto | Bray et al. Nature Biotechnology. (2016) | |
| DESeq2 | Love et al. Genome Biology. (2014) | |
| fgsea | Sergushichev A. bioRxiv (2016) | |
| EnhancedVolcano | Blighe K. (2018) | |
| Other | ||
| RPMI 1640 | Thermo | 21870092 |
| HEPES Buffer | Gibco | 15630 |
| L-glutamine | Gibco | 25030 |
| Fetal Bovine Serum (FBS) | Gibco | Gibco-Performance 26140-079 |
| β-mercaptoethanol | Sigma-Aldrich | M3148 |
| Penicillin-streptomicin | Gibco | 15140122 |
| Scepter cell counter | Millipore | PHCC40500 |