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. 2021 Jun 19;14:28. doi: 10.1186/s13072-021-00401-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The EPCAM promoter region as a representative example for data generated with the protocols. The aligned reads tracks are taken from the Integrated Genomics Viewer (IGV) [45] in the bisulfite mode where red represents an unconverted cytosine and blue represents a converted cytosine. Each panel represents one sample, with A and B denoting the biological replicates and 1 and 2 the technical replicates for each library construction protocol. The shown region is 1500 bp upstream and downstream of exon 1. The location of a CpG island is indicated with a green box on the bottom. Note, the strands for the PBAT samples have been flipped in silico before being displayed to account for the strand definition in the Miura and Ito protocol. The strands in the PBAT protocol are opposite from what is expected by IGV, as well as the definition used by the other protocols. All libraries were downsampled to be comparable to PBAT; therefore, any differences are not likely confounded by sequencing depth. As expected, this coverage will be substantially higher at increased depth