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. 2021 Jun 19;14:28. doi: 10.1186/s13072-021-00401-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Principal components of average methylation levels in 100 kb bins. Principal component 1 accounts for 41.4% of the variance and principal component 2 accounts for 21.6%. The four protocols are presented with different colors. Biological replicates are displayed with shapes, while technical replicates are shown with different empty or filled markers. With regards to the two Kapa Sample B technical replicates, these points sit almost on top of one another, making them hard to distinguish at the scale shown. Note, all libraries were downsampled to be comparable to PBAT