Fig 2.

Pseudoalteromonas luteoviolacea HI1 wild type produces TBP, while the Δbmp2 strain does not. (A) Genomic arrangement of the bmp gene cluster in strain HI1. Bolded genes bmp1–4 have previously been validated to code for TBP biosynthesis. The blue bolded gene bmp2 was deleted to create a nonfunctional TBP mutant in P. luteoviolacea. The gene outlined in red bmp8 is a pseudogene. (B) Representative ion chromatogram (EIC = 381.67) overlaid the comparison of an organic extraction of wild type and Δbmp2 strains to the TBP standard. Cultures were grown in MB and SWT media overnight in triplicate to quantify TBP production in P. luteoviolacea (see Fig. S1a).