Fig 4.

Pseudoalteromonas luteoviolacea MACs stimulate the metamorphosis of Hydroides larvae.

(A) Schematic of larval and metamorphosis phenotypes scored for Hydroides. Metamorphosis assays are performed as described previously (Shikuma et al., 2014). Phenotypic response of Hydroides larvae to treatments containing (B) increasing concentrations of purified TBP and (C) mutant biofilms. The bars represent the average of (B) five biological replicates (n = 5) and (C) three biological replicates (n = 3). A statistical power analysis aided with the determination for the appropriate number of biological replicates. Values for the biological replicates were found by averaging four technical replicates per treatment. The biological replicates were performed on different batches of larvae on different days. Error bars denote standard deviation. Asterisks above the bars denote statistical significance compared to the (B) ACN and (C) ASW controls. Statistical analyses were performed with a (B) Kruskal–Wallis ANOVA and (C) one-way ANOVA both corrected for multiple comparisons by FDR using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (GraphPad Prism), where ***p = 0.0054, and ****p < 0.0001.