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. 2021 Jun 8;10(6):bio058008. doi: 10.1242/bio.058008

Fig. 1.

Fig. 1.

Anti-MICAL-L1 antibodies specifically recognize the protein. (A) Equal amounts of proteins (30 µg) prepared from HeLa cells and from HeLa expressing scramble (Scr) and two shRNAs targeting MICAL-L1 were analyzed by immunoblot using affinity purified anti-MICAL-L1 antibodies. Tubulin was used as loading controls. (B) HeLa cells on coverslips expressing Scr and shRNAs targeting MICAL-L1 were fixed and incubated with rabbit affinity purified anti-MICAL-L1 antibody followed by staining with Alexa Fluor 547-conjugated goat anti-rabbit antibody. Scale bar: 10 µm.