Fig. 4.

MICAL-L1 controls membrane trafficking. HeLa cells expressing Scr and two different shRNAs targeting MICAL-L1 were transfected with TNFα-SBP-mCherry, and cadherin-mCherry, using the RUSH system. After addition of 40 µM Biotin, cells were stained with anti-GM130 and analyzed by immunofluorescence at 0, 30 and 60 min. 3D-projections of images are shown. Note that depletion of MICAL-L1 delayed cargo transport to the PM. Scale bars: 10 µm. Quantification of post-Golgi vesicles was performed as indicated in the Materials and Methods section.