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. 2021 Jun 8;10(6):bio058222. doi: 10.1242/bio.058222

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Overview of DEGs related to transcription factors, WNT signalling and extracellular matrix related genes, differentially expressed between the first and second trimester. (A) Heatmap of DEGs associated with WNT signalling. (B) Heatmap of extracellular matrix and angiogenesis-related DEGs. (C–E) Immunolocalisation of meprin (D), CD31 (E) and musculin (MSC) (F) in first- and second-trimester placental sections (n=4). Scale bars are 25 µm in C and E and 50 µm in D. (F) Heatmap of differentially expressed transcription factors. (G) CBX3 motif is enriched in proportion of DEGs: sequence motif for CBX3, incidence matrix of highly ranked DEGs with CBX3 motif, and their pathway enrichment analysis.