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. Author manuscript; available in PMC: 2021 Jun 19.
Published in final edited form as: FASEB J. 2020 Jun 4;34(7):9433–9449. doi: 10.1096/fj.201800641R

FIGURE 2.

FIGURE 2

Mov10 inhibits HCV RNA production from fully infectious virus and subgenomic replicon. A, To determine the effect of Mov10 overexpression on HCV RNA levels, Huh7.5.1 cells were electroporated with Jc1-FLAG2(p7-nsGluc2A) RNA (10 μg). About 16 hpe cells were transfected with 0.4 μg of control (pUC18), mRFP-DCP1a, or mRFP-Mov10 plasmids. At 24, 48 and 72 hpt total RNA was isolated from the cells (intracellular HCV RNA) and analyzed for HCV RNA levels by RT-qPCR. B, The levels of DCP1a and Mov10 expression were quantified by flow cytometry and presented by percent of mRFP cells. C, SG-neo (wt) replicon RNA (1 μg) was transfected into Huh7.5.1 cells by electroporation. About 16 hpe cells were transfected with either a plasmid expressing mRFP-DCP1a or mRFP-Mov10 (0.1, 0.2, 0.3, or 0.4 μg). Cells were collected at 48 hpt and analyzed for HCV RNA levels by RT-qPCR. D, The levels of DCP1a and Mov10 expression were quantified by flow cytometry and presented by percent of mRFP cells. Data were normalized to data for the empty plasmid control. Each data point represents average value of 3 individual experiments. Error bars represent SD