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. Author manuscript; available in PMC: 2021 Jun 19.
Published in final edited form as: FASEB J. 2020 Jun 4;34(7):9433–9449. doi: 10.1096/fj.201800641R

FIGURE 8.

FIGURE 8

Rescue of HCV replication by exogenous expression of Mov10. D6 cells that have Mov10 suppressed by CRISPR/Cas9 were electroporated with Jc1-FLAG2(p7-nsGluc2A) RNA. A 16 hpe cells were transfected with varying amounts (0.1, 0.2, 0.3, and 0.4 μg) of an mRFP-Mov10 expression plasmid. A, 72 hpt cells were lysed and assayed for Mov10 expression and HCV core by western blot. A 1 is exogenous mRFP-Mov10 and 2 is endogenous Mov10. B, Cell culture media were assayed for Gaussia luciferase activity at 72 hpt. Data are normalized to the parental cell line Huh7.5.1. Each data point represents average value of 3 individual experiments. Error bars represent SD. Statistical analysis was performed by using Student’s t-test (*P < .05, **P < .01)