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. 2021 May 27;118(24):e2101161118. doi: 10.1073/pnas.2101161118

Fig. 6.

Fig. 6.

NSP14 inhibits the protein expression of ISGs. (A) Vero E6 cells were transfected with plasmids encoding NSP1 or NSP14 for 24 h and treated with IFN-I for 18 h. The expression of IFN-stimulated proteins was determined by immunoblotting with antibodies against depicted proteins. (B) The induction of ISGs on mRNA level from A were determined by qRT-PCR with primers against genes encoding RIG-I and viperin proteins (RSAD2). (C) The 293T cells were transfected with HA-tagged NSP14 for 24 h and treated with IFN-I for 18 h. The induction of ISGs was determined by immunoblotting with antibodies against depicted proteins. Asterisk (*) indicates nonspecific protein bands. (D) The 293T cells were transfected with plasmids encoding NSP1 or NSP14 for 24 h and treated with IFN-I for 18 h. The expression of IFN-stimulated proteins of ISGs was determined by immunoblotting with antibodies against depicted proteins. For B, data are shown as mean ± SD of six biological repeats by unpaired Student’s t test.