Fig. 4.

Myeloid cell types detected through single-cell analyses of ccRCC and their associations with survival. (A) t-SNE plot depicts the seven major myeloid lineage cell types captured by scRNA-seq: macrophages-A and -B, monocytes-A and -B, and dendritic cells (DC)-A, -B, and -C. (B) Stacked bar plot shows samplewise frequency/composition of the myeloid cell types. Myeloid cell numbers in general are much higher in tumors compared with adjacent normal tissues (bar plot, Right). Macrophage-B population is enriched in tumors compared with normal. (C) Significantly enriched KEGG pathways (FDR < 0.05) in macrophage-B vs. macrophage-A population comparison. Light blue bars: positively enriched in macrophage-A; dark blue bars: positively enriched in macrophage-B. (D) Survival plots (TCGA-ccRCC bulk RNA-seq data) based on signature genes that are differentially expressed between macrophage-A and macrophage-B populations.