Fig. 6.

An anti-mouse CD81 antibody inhibits breast cancer migration in vitro and reduces metastasis in vivo. (A) 4T1-luc cells were place at the top chamber in a Transwell assay and incubated with the indicated anti-mouse CD81 mAb (α-MsCD81) and its respective isotype control. Migrating cells attached at the bottom well were stained with crystal violet and quantified. Representative migrated cells are shown. Shown are mean ± SEM, Student’s t test, P = 0.0001***. (B) Schema: Mice were injected with 400 µg of anti-mouse CD81 on day −1 and with 400 µg on days +1 and +3; on day 0 mice were injected with 2.5 × 10⁴ 4T1-luc intravenously; on day 16 mice were killed and lung metastasis was visualized (C, Left) by bioluminescence (IVIS imaging) and quantified (C, Right) by India ink staining (n = 20 mice in each group, three independent experiments). Shown are individual mouse metastatic nodule counts, Student’s t test, P = 0.0065**.