Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 1;14(5):dmm044669. doi: 10.1242/dmm.044669

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 5. — JNK and Drpr regulate each other and this requires Grnd. (A-B″) Representative metathoracic neuromeres (MtNs) stained for Drpr, showing the TRE-RFP reporter signal at 24 h ACI in control flies (A,B) and in flies with knocked down drpr (A′,B′) or knocked down grnd (A″,B″) in glial (repoGal4) cells. (C) Quantification of MtN:PN ratio of Drpr staining. One-Way ANOVA, *P<0.05; Bonferroni multiple comparison, ***P<0.0001. (D) Quantification of MtN:PN ratio of the TRE-RFP reporter signal. One-Way ANOVA, **P<0.001; Bonferroni multiple comparison, ***P<0.0001. (E) Images show the accumulation of nuclear debris (arrowheads, stained with DAPI, grey) due to downregulation of drpr expression in glial cells. The TRE-RFP JNK reporter is shown in red, glial cells (repo>GFP) are shown in green. (F,G) Quantification of the metathoracic neuromere to prothoracic neuromere (MtN:PN) ratios of the TRE-RFP mean grey value of not injured to injured VNCs after knocking down grnd or eiger in glial cells (repoGal4, F) or neurons (elavGal4, G). Dunn's multiple comparison test, *P<0.05, **P<0.001, ***P<0.0001. ns, not significant. Genotypes: TRE-RFP; repoGal4-UAS-GFPnls (A-D). TRE-RFP; repoGal4>UAS-GFPnls, UAS-drprRNAi (A′,B′,C-E). TRE-RFP; repoGal4>UAS-GFPnls, UAS-grndRNAi (A″,B″,C,D). TRE-RFP; repoGal4>UAS-GFPnls, TRE-RFP; repoGal4>UAS-GFPnls, UAS-grndRNAi, TRE-RFP; repoGal4>UAS-GFPnls, UAS-eigerRNAi (F). TRE-RFP; elavGal4>+, TRE-RFP; elavGal4>UAS-grndRNAi, TRE-RFP and elavGal4>UAS-eigerRNAi (G). Scale bars: 50 μm (A,B), 15 μm (E).