Skip to main content
. 2021 Jun 7;134(11):jcs258437. doi: 10.1242/jcs.258437

Fig. 1.

Fig. 1.

Both minus-end-out and plus-end-out microtubules are generated at dendrite branch points. (A) Overview image of the Drosophila Class I neuron ddaE expressing UAS-Eb1-GFP using 221-Gal4. (B) Left: example of a ddaE dendrite segment labeled by Eb1-GFP. The yellow line circles the branch point area in which nucleation direction was monitored. The cyan line indicates the region in which the kymograph on the right was generated. (C) Quantification of Eb1-GFP comet traveling direction in main dendrite (between branch points) and within dendrite branch points. Numbers on the graph are the total comets analyzed. ****P<0.0001 (Fisher's exact test). (D) Schematic diagram of nucleation direction and overall microtubule polarity in dendrites. Darker gray area indicates a branch point.