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. 2021 Jun 7;134(11):jcs258437. doi: 10.1242/jcs.258437

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© 2021. Published by The Company of Biologists Ltd

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Fig. 5. — Eb1-TagRFP-T recruits Trim9 to microtubules in S2R+ cells. (A) UAS-Eb1-TagRFP-T was expressed in S2R+ cells under Actin-Gal4. αTubulin was visualized by antibody staining. (B) UAS-mNG-Trim9-RA was expressed in S2R+ cells with Actin-Gal4, and αTubulin was visualized with an antibody. (C) Cells with mildly overexpressed or highly overexpressed Eb1-TagRFP-T. UAS-mNG-Trim9-RA and UAS-Eb1-TagRFP-T were expressed in S2R+ cells with Actin-Gal4. Arrows indicate colocalization of mNG-Trim9-RA and Eb1-TagRFP-T. (D) Line intensity traces of Eb1-TagRFP-T, αTubulin antibody staining or mNG-Trim9-RA were generated from the images below. The blue lines on the images indicate the lines used to generate fluorescent intensity (FI) in the graphs. Scale bars: 5 μm.