Fig. 1.

Atg32-dependent mitophagy is necessary for respiratory growth during heat stress. (A-B) Yeast serial dilutions grown on YPD and YPGE media at 30°C and 37°C (n=3). (C) Immunoblot of Om45-GFP lysosomal turnover from strains cultured at 30°C in SGE or YPGE media, and shifted to heat stress [SGE (n=4) or YPGE (n=3), 37°C] or nitrogen starvation 30°C (SD-N) (n=4). (D-F) Serial dilutions of yeast grown on YPGE at 30°C and 37°C (n=3). WT, wild type. The center line denotes the median value (50th percentile), while the box contains the 25th to 75th percentiles of the dataset. The black whiskers with a dot at the end of a line mark the 5th and 95th percentiles, and values beyond these upper and lower bounds are considered outliers, marked with black dots.