Fig. 3.

SAM and spermidine are necessary and sufficient to restore respiratory growth of atg32Δ cells during heat stress. (A-B) Serial dilutions of yeast spotted on YPD and YPGE plates with or without 0.2 mM SAM (A) or 0.1 mM spermidine (B) and grown at 37°C. (C-D) Serial dilutions of indicated yeast strains on YPGE plates at 30°C and 37°C. (E) Om45-GFP lysosomal turnover measured by immunoblot analysis from the indicated yeast cultured at 30°C in YPGE medium and shifted to 37°C. WT, wild type. The center line denotes the median value (50th percentile), while the box contains the 25th to 75th percentiles of the dataset. The black whiskers with a dot at the end of a line mark the 5th and 95th percentiles, and values beyond these upper and lower bounds are considered outliers, marked with black dots.