KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-BrdU | Abcam | #ab6326; RRID: AB_305426 |
| Goat polyclonal anti-mouse ACE2 | R&D Systems | #AF3437; RRID: AB_2223140 |
| Mouse monoclonal anti-ACTA2-Cy3 | Sigma-Aldrich | #C6198, RRID: AB_476856 |
| Mouse monoclonal anti-AQP1 | Santa Cruz | #32737; RRID: AB_626692 |
| Rabbit polyclonal anti-CCDC67 | Proteintech | #24579-1-AP |
| Goat polyclonal anti-CD62P/P-Selectin | R&D Systems | #AF737; RRID: AB_2285644 |
| Rat monoclonal anti-CD74-488 | Biolegend | #151005; RRID: AB_2750325 |
| Mouse monoclonal anti-CLDN5-488 | ThermoFisher | #352588; RRID: AB_2532189 |
| Rabbit polyclonal anti-COL1A1 | Abcam | #ab34710, RRID: AB_731684 |
| Goat polyclonal Anti-ESM1 | R&D Systems | #AF1999, RRID: AB_2101810 |
| Chicken polyclonal anti-GFP | Abcam | #ab13970; RRID: AB_300798 |
| Rabbit polyclonal anti-GFAP | Dako | #Z0334; RRID: AB_10013382 |
| Rabbit polyclonal anti-IBA1 | Wako | #019-19741, RRID: AB_839504 |
| Hamster monoclonal anti-IL1B | Biolegend | #503513, RRID: AB_2814399 |
| Goat polyclonal anti-IL1R1 | R&D Systems | #AF771, RRID: AB_355587 |
| Mouse monoclonal anti-KI67 | BD PharMingen | #550609; RRID: AB_393778 |
| Rat monoclonal anti-KI67 | ThermoFisher | #14-5698-80; RRID: AB_10853185 |
| Goat polyclonal anti-LYVE1 | R&D Systems | #AF2125, RRID: AB_2297188 |
| Rabbit polyclonal anti-MRC1/CD206 | Abcam | #ab64693, RRID: AB_1523910 |
| Goat polyclonal anti-NRP1 | R&D Systems | #AF566; RRID: AB_355445 |
| Rabbit polyclonal anti-PAX6 | Millipore | #ab2237, RRID: AB_1587367 |
| Rat monoclonal anti-PECAM1/CD31 | BD PharMingen | #550274, RRID: AB_393571 |
| Rabbit polyclonal anti-SHISA8 | Abcam | #ab188621 |
| Rat monoclonal anti-SPI1/PU.1 | Novus Bio | #MAB7124-SP |
| Rabbit polyclonal anti-SPIC | ThermoFisher | #PA5-67537, RRID: AB_2692238 |
| Rabbit anti-SLC40A1 | Alpha Diagnostic International | #MTP11-A, RRID: AB_1619475 |
| Mouse monoclonal anti-TUBB3 | Biolegend | #801202, RRID: AB_10063408 |
| Mouse monoclonal anti-Acetylated Tubulin | Sigma-Aldrich | T6793, RRID: AB_477585 |
| Rabbit polyclonal anti-VWF | ThermoFisher | #MA5-14029; RRID: AB_305689 |
| Rat monoclonal anti-WNT5A | R&D Systems | #MAB645; RRID: AB_10571221 |
| Mouse monoclonal anti-ZO1 | ThermoFisher | #33-9100; RRID: AB_2533147 |
| Rabbit polyclonal anti-5HT | Sigma-Aldrich | #S5545; RRID: AB_477522 |
| Chemicals, peptides, and recombinant proteins | ||
| Collagenase II | GIBCO | 17101-015 |
| TrypLE | Life Technologies | 12604 |
| LIVE/DEAD staining kit | ThermoFisher | L-3224 |
| 10% Tween-20 (used for TST) | VWR Teknova | T0710 |
| NP-40 Surfact-Amps Detergent Solution (used for NST) | ThermoFisher | 28324 |
| CHAPS, Molecular Biology Grade, (used for CST) | EMD Millipore | 220201-1GM |
| Nuclei EZ Prep Nuclei Isolation Kit | Sigma | NUC101-1KT |
| Critical commercial assays | ||
| 10X Genomics Single Cell 3′ Gel Bead Kit V1 | 10X Genomics | PN-120231 |
| 10X Genomics Single Cell 3′ Gel Bead Kit V2 | 10X Genomics | PN-120237 |
| 10X Genomics Single Cell 3′ Gel Bead Kit V3 | 10X Genomics | PN-1000075 |
| RNAscope Fluorescent Multiplex | ACD | #320851 |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO: GSE168704 |
| Experimental models: Organisms/strains | ||
| Mouse: CD-1 (ICR) | Charles River Laboratories | Strain 022 |
| Mouse: C57BL/6J | Jackson Laboratory | 000664 |
| Mouse: C57BL/6 | Charles River Laboratories | N/A |
| Mouse: B6.129P2(Cg)-Cx3cr1tm1Litt/J | Jackson Laboratory | 005582 |
| Mouse: 129S4.Cg-E2f1Tg(Wnt1-cre)2Sor/J | Jackson Laboratory | 002137 |
| Mouse: Gt(ROSA) 26Sortm1(CAG-Brainbow2.1)Cle/J | Jackson Laboratory | 013731 |
| Oligonucleotides | ||
| mm-Axin2-C3 | ACD | 400331-C3 |
| mm-FoxJ1 | ACD | 317091 |
| mm-Ins2-C2 | ACD | 310751-C2 |
| mm-Rspo1 | ACD | 401991 |
| mm-Rspo2-C2 | ACD | 402001-C2 |
| mm-Rspo3-C3 | ACD | 402011-C3 |
| mm-Wnt1 | ACD | 401091 |
| mm-Wnt2b-C2 | ACD | 405031-C2 |
| mm-Wnt3a | ACD | 405041 |
| mm-Wnt5a-C3 | ACD | 316791-C3 |
| Software and algorithms | ||
| Cellranger | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome | N/A |
| CellPhoneDB | Efremova et al., 2020 | N/A |
| Seurat | https://github.com/satijalab/seurat | N/A |
| R | https://www.r-project.org/ | N/A |
| Other | ||
| Noyes Spring Scissors – Tungsten Carbide/Straight | Fine Science Tools | #15514-12 |
| Dounce Tissue Grinder Set | Sigma | #D8938-1SET |