Fig. 5. Fedratinib combined with trametinib suppresses tumor growth and induces apoptosis in MGC803 gastric cancer (GC) cell xenograft tumors in vivo. (a) Mice with tumors formed by subcutaneous injection of MGC803 GC cells received fedratinib and trametinib alone or in combination (n=6). (b) Resected tumor tissues after the in vivo experiment are shown (n=6). (c) Tumor growth curve was documented based on tumor volume on the indicated following days. (d) Weights of tumors exposed to fedratinib and trametinib alone or in combination were examined on the indicated days (n=6). (e) Apoptotic level was analyzed using immunoblotting of cleaved caspase-9 in tumor tissue lysates. The results are presented as mean±standard deviation (SD). ^* P<0.05 and ^** P<0.01 vs. control; ^& P<0.05 vs. 0.75 mg/kg trametinib treatment; ^# P<0.05 and ^## P<0.01 vs. 30 mg/kg fedratinib treatment.