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. 2021 May 6;101(7):908–920. doi: 10.1038/s41374-021-00577-7

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© The Author(s), under exclusive licence to United States and Canadian Academy of Pathology 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Expression of tumor stem cell maker CD133⁺CD44⁺ in human colon cancer HCT116 cells detected by flow cytometry. B Expression of SLCO4A1 in colon epithelial cells NCM460, HCT116 and CD133⁺CD44⁺ cells determined by RT-qPCR, expression of SLCO4A1 normalized to GAPDH, *p < 0.05 vs. colon epithelial cells NCM460, **p < 0.01 vs. HCT116 cells. The data were the measurement data and expressed as mean ± standard deviation. One-way ANOVA was used for the data analysis among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times.