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. 2021 May 6;101(7):908–920. doi: 10.1038/s41374-021-00577-7

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© The Author(s), under exclusive licence to United States and Canadian Academy of Pathology 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The localization of SLCO4A1-AS1A in cells detected by FISH. B The binding site between miR-150-3p and SLCO4A1-AS1 predicted using the online prediction software. C The relationship between miR-150-3p and SLCO4A1-AS1 detected by dual-luciferase reporter assay. *p < 0.05 vs. NC treatment. D Relative enrichment of SLCO4A1-AS1 by miR-150-3p detected by RNA pull-down. *p < 0.05 vs. Mut-bio-miR-150-3p. E SLCO4A1-AS1 and miR-150-3p binding to Ago2 protein determined by RIP. ^#p < 0.05 vs. IgG. The data were the measurement data and expressed as the mean ± standard deviation. Unpaired t-test was applied for the comparison between two groups while one-way ANOVA was used among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times.