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. 2021 May 6;101(7):908–920. doi: 10.1038/s41374-021-00577-7

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© The Author(s), under exclusive licence to United States and Canadian Academy of Pathology 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A SLCO4A1-AS1 expression in CD133⁺CD44⁺ cells and HCT116 cells detected by western blot assay. B The migration ability of CD133⁺CD44⁺ cells detected by scratch test. C The invasion ability of CD133⁺CD44⁺ cells detected by transwell assay. D The sphere formation ability of CD133⁺CD44⁺ cells detected by suspension sphere formation assay. E The apoptosis of colon cancer cell CD133⁺CD44⁺ cells detected by flow cytometry. F The weight of tumors of nude mice. G The volume of tumors of nude mice. H SLCO4A1-AS1 expression in tumors of nude mice detected by western blot assay. *p < 0.05 vs. cells co-transfected with oe-NC and miR-150-3p mimic. The data were the measurement data and expressed as the mean ± standard deviation. Unpaired t-test was applied for the comparison of data between two groups. The cell experiment was repeated three times. Repeated measurement ANOVA was applied for data comparison between groups at different time points with Bonferroni’s post hoc test. n = 8. The cell experiment was repeated three times.