Fig. 4.

Induction of the carbonyl reductase gene in Mytilus spp. gills after a 96-h treatment with TNT—representative agarose gel. Mussels were exposed to increasing concentrations of TNT (ranging from 0.31 to 10 mg/L) for 96 h in laboratory aquarium studies. Exposed mussels plus control specimens (without TNT exposure) were dissected and tissues samples were processed for mRNA extraction. The primers used for RT-PCR are shown in Table 1. 18 s RNA served as the housekeeping gene