Fig. 6.

Induction of the carbonyl reductase in Mytilus spp. tissues after a 21-day treatment with TNT—densitometric analyses. Mussels were exposed to increasing concentrations of TNT (ranging from 0.31 to 2.5 mg/L) for 21 days in laboratory aquarium studies. Exposed mussels plus control specimens (without TNT exposure) were dissected and tissues samples were processed for mRNA extraction. The primers used for RT-PCR are shown in Table 1. 18 s RNA served as the housekeeping gene. Fold inductions (means with SD from n = 3–9) were calculated from the carbonyl reductase expression relative to 18 s RNA. Statistically significant changes are indicated by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Dunnett’s T3 multiple comparisons test was used for the calculation of the gills and hepatopancreas results, Dunn’s multiple comparisons test for the calculation of the mantle and muscle results)