Fig. 1.

Inhibition of mTORC2 sensitizes NSCLC cells to gemcitabine treatment. (A) H460, H1975 and H157 cells were treated with 0.5 µM gemcitabine, 1 µM Rapamycin, 1 µM PP242 or their combination as indicated. Cell apoptosis were analyzed by Annexin V staining at 48 hrs after treatment. (B) Knockout (KO) of Rictor in H1299 and H460 cells using CRISPR/Cas9, and western blot analysis of expression of indicated proteins. (C) Colony formation survival analysis of H460 and H1299 parental or Rictor KO cells in presence or absence of 3 nM gemcitabine. Relative number of colonies were quantified and normalized to untreated parental cells (right) and representative colony formations were shown (left). * P < 0.05, **P < 0.01 and *** P < 0.001 by two-tailed t-test.