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. 2021 Jun 5;7(6):e07237. doi: 10.1016/j.heliyon.2021.e07237

Table 1.

Composition analyzes of the Phytase HM preparation for the toxicological studies.

Composition Limits Unit
Enzyme activity - FYT/g 34000
Water (Karl Fisher) - % w/w 88.0
Total Organic Solids - % w/w 10.5
Dry Matter - % w/w 12
Ash (600C) - % w/w 1.5
N-total - % w/w 1.18
Carbohydrate (anthron) - g/kg 24.2
Carbohydrate (tryptophan) - g/kg 36.4
Water at 105 °C - % w/w 88
Heavy Metals(1) ≤30 mg/kg mg/kg 4.3
Coliforms ≤30 /g <4
Escherichia coli ND /25g ND
Bacillus cereus ≤102 /g <10
Sulphite red. clostridia ≤30 /g <10
Staphylococcus aureus ND /g ND
Salmonella sp. ND /25g ND
Antimicrobial activity ND - ND
Toxins(2) <LOD mg/kg <LOD
Production organism (Aspergillus oryzae) ND /g ND

ND = not detected.

(1)

Heavy Metals = ΣDLT of Ag, As, Bi, Cd, Co, Cu, Hg, Mo, Ni, Pb, Sb, Sn.

(2)

Aflatoxin B1, Cyclopiazonic acid, Beta-nitropropion acid and Kojic acid.