Table 10.
3-hour treatment in the presence of S9 mix.
| Treatment/Concentration (μg/mL)ˆ | Mean Cytostasis (%) | Binucleated cells containing micronuclei |
|||
|---|---|---|---|---|---|
| per 1000 cells$ | Mean | p-valueb | Trend test | ||
| p-valuec | |||||
| Vehiclea | 0.0 | 6, 8 | 6.8 | ||
| 8, 9 | |||||
| 6, 4 | |||||
| 7, 6 | |||||
| Phytase HM/1250 | 1.2 | 5, 7 | 5.0 | 1.000 | |
| 5, 3 | |||||
| Phytase HM/2500 | 4.4 | 6, 9 | 7.8 | 0.551 | 0.295 |
| 8, 8 | |||||
| Phytase HM/5000 | 2.5 | 8, 5 | 7.3 | 0.551 | 0.160 |
| 9, 7 | |||||
| CPA/10 | 47.3 | 20, 17 | 18.5 | <0.001∗∗∗ | |
| 18, 19 | |||||
No statistically significant increases in the induction of micronuclei were seen after three hours exposure with S9 and no dose response relationship was found.
ˆ Test item concentrations are reported in terms of Total Organic Solids (TOS);
$Values presented from individual slides; underlined values obtained from additional analysis;
a. Vehicle control = Water (10% v/v);
b. p-values are for comparisons to control using Williams' test and t-tests;
c. Trend test p-values are for the linear contrast including the control group and lower concentrations of the same test item.
∗∗∗p < 0.001.
CPA: Cyclophosphamide.