Table 11.

20-hour treatment in the absence of S9 mix.

Treatment/Concentration (μg/mL)ˆ	Mean Cytostasis (%)	Binucleated cells containing micronuclei
		per 1000 cells	Mean	p-valueb	Trend test
					p-valuec
Vehiclea	0.0	7	5.8
		4
		5
		7
Phytase HM/1250	1.4	5	4.5	1.000
		4
Phytase HM/2500	5.7	6	5.5	1.000	0.853
		5
Phytase HM/5000	14.7	6	6.5	0.562	0.303
		7
MMC/0.1	21.1	20	19.5	<0.001∗∗∗
		19
COL/0.015	58.1	17	18.5	<0.001∗∗∗
		20

No statistically significant increases in the induction of micronuclei were seen after 20 h exposure without S9 and no dose response relationship was found.

ˆ Test item concentrations are reported in terms of Total Organic Solids (TOS);

a. Vehicle control = Water (10% v/v);

b. p-values are for comparisons to control using Williams' test and t-tests;

c. Trend test p-values are for the linear contrast including the control group and lower concentrations of the same test item.

∗∗∗p < 0.001.

MMC: Mitomycin C; COL: Colchicine.