Table 8.
Bacterial reverse mutation assay.
| Compound | μg/plate | S9 | TA98 | TA100 | TA1535 | TA1537 | WP2 uvrA pKM101 |
|---|---|---|---|---|---|---|---|
| Purified water | - | - | 53.3 | 158.3 | 25 | 13.3 | 150.7 |
| Phytase HM | 50 | - | 37.0 (0.7) | 179.7 (1.1) | 25.3 (1.0) | 13.7 (1.0) | 119.3 (0.8) |
| Phytase HM | 150 | - | 55.0 (1.0) | 153.3 (1.0) | 22.0 (0.9) | 10.3 (0.8) | 128.0 (0.8) |
| Phytase HM | 500 | - | 51.3 (1.0) | 167.0 (1.1) | 30.0 (1.2) | 12.7 (0.9) | 124.3 (0.8) |
| Phytase HM | 1500 | - | 44.3 (0.8) | 179.7 (1.1) | 28.0 (1.1) | 11.7 (0.9) | 129.7 (0.9) |
| Phytase HM | 5000 | - | 55.7 (1.0) | 207.0 (1.3) | 19.7 (0.8) | 14.0 (1.0) | 142.3 (0.9) |
| 2NF | 1.25 | - | 174.3 (3.3) | ||||
| NQO | 0.3 | - | 1278.7 (8.1) | ||||
| NaN3 | 1.9 | - | 85.0 (3.4) | ||||
| AAC | 31.3 | - | 71.0 (5.3) | ||||
| NQO | 0.3 | - | 1028.3 (6.8) | ||||
| Purified water | - | + | 51 | 178.3 | 25 | 10 | 153.7 |
| Phytase HM | 50 | + | 57.0 (1.1) | 147.0 (0.8) | 24.0 (1.0) | 8.3 (0.8) | 141.3 (0.9) |
| Phytase HM | 150 | + | 51.3 (1.0) | 146.3 (0.8) | 24.7 (1.0) | 10.3 (1.0) | 158.0 (1.0) |
| Phytase HM | 500 | + | 55.3 (1.1) | 154.7 (0.9) | 25.3 (1.0) | 10.3 (1.0) | 159.0 (1.0) |
| Phytase HM | 1500 | + | 41.7 (0.8) | 163.3 (0.9) | 23.7 (0.9) | 9.0 (0.9) | 170.7 (1.1) |
| Phytase HM | 5000 | + | 55.3 (1.1) | 164.7 (0.9) | 24.3 (1.0) | 7.3 (0.7) | 164.3 (1.1) |
| B[a]P | 9.4 | + | 152.3 (3.0) | ||||
| AAN | 6.3 | + | 1337.0 (7.5) | 148.0 (5.9) | |||
| AAN | 9.4 | + | 120.7 (12.1) | 425.0 (2.8) |
Mean number of revertant colonies per plate. Fold increase in revertants in treated cells relative to vehicle is reported in the parentheses. The fold increase ranges from 0.7 to 1.2 demonstrating no mutagenic potential of Phytase HM. Abbreviations; TOS: Total Organic Solids, NaN3: Sodium azide, 2-NF: 2-Nitrofluorene, NQO: 4-Nitroquinoline-1-oxide, AAC: 9-Aminoacridine, B[a]P: Benzo[a]pyrene, AAN: 2-Aminoanthracene.