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Two-plasmid reporter assay. E. coli strains containing the empty pBAD33 plasmid or the indicated opaR expression plasmid pBAD33-opaR and one of the recombinant lacZ plasmids were grown in LB broth containing 0.2% arabinose to mid-log phase (OD600 = 1.2), at which point aliquots were collected and assayed for lacZ expression using the β-galactosidase assay. The negative and positive numbers indicate the nucleotide positions upstream and downstream of the indicated genes, respectively.
