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. 2021 Jun 7;12:676436. doi: 10.3389/fmicb.2021.676436

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Copyright © 2021 Zhang, Qiu, Gao, Sun, Li, Zhang, Xue, Yang, Ni, Hu, Yin, Lu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription start sites for target genes. Negative numbers represent nucleotide positions upstream of the translation start site for each target gene. The transcription start sites are marked with arrows and positions. Lanes G, A, T, and C represent the Sanger sequencing reactions. (A) Primer extension. An oligonucleotide primer complementary to the mRNA of each target gene was designed. The primer extension products were analyzed with an 8 M urea-6% acrylamide sequencing gel. (B) 5′ RACE. The 5′-end of transcripts of target genes were analyzed using the FirstChoice^®RLM-RACE (Invitrogen, United States) according to the manufacturer’s instructions.