TABLE 1.

Oligonucleotide primers used in this study.

Target	Primers (forward/reverse, 5′–3′)
Protein expression
opaR	AGCGGGATCCATGGACTCAATTGCAAAGAG/AGCGAAGCTTTTAGTGTTCGCGATTGTAG
qPCR
scrA	CACACCACGAACACATTGC/TCAATAGCGTCACGGAATGC
scrG	AAGCCGTGGTGGAAGAAGG/GCGTGTTGAGTGCGTTGG
VP0117	GACCACCTCAATAGTTATCTG/TAAGTAGGCTTGGACATCTC
VPA0198	GCATCAGAATCAGCAAGAC/ATGCTTAGCTCCTCTTCTTC
VPA1176	GCCATATTCCAAACTCGTTGTG/TGCGTAAGCCAAGTTGATGAG
VP0699	CTGACACATCGTGATACTTC/TTGATGTTGCAGCTCTTG
VP2979	GCAACTCTCAAGTCATCATC/CAACAACCGTCTTCTATGG
Primer extension
scrA	/GACTTTAGTTCCACTTTTTTAGC
scrG	/ACTTAGTCAACAGTAAATCGTG
VP0117	/GACAATCACACCGATAGCCAG
VPA1176	/CACCCCATTCTGTCCAT
LacZ fusion
scrA	GCGCGTCGACCATCAAGCCATTTTATGAAAC/GCGCGAATTCGTCGGCTGCGATTAGTCTG
scrG	GCGCGTCGACTAGCACGCTTGTGTTGGAC/GCGCGAATTCCAGGGAAATGAAGTAATCATGC
VP0117	GCGCTCTAGACTCACACAACACTTTCTG/GCGCGAATTCAGACAATCACACCGATAG
VPA0198	GCGCGTCGACCTCTGGTTCATTGTCTTG/GCGCGAATTCGTCTTGCTGATTCTGATG
VPA1176	AAAGTCGACTCAGGTACGCTTGCTTCAC/GGGGAATTCCGTTGCTTGGTAGTGGTAATAG
VP0699	GCGCGTCGACGGAGAATACCTAGCAGAG/GCGCGAATTCAGTATCACGATGTGTCAG
VP2979	GCGCTCTAGATTTCTATCCGTTGGCTAC/GCGCGAATTCCTGACTTACATCGTGGAC
EMSA
scrA	GAGCGTATATCCAAGTGGTTTG/GTCGGCTGCGATTAGTCTG
scrG	TAGCACGCTTGTGTTGGAC/CAGGGAAATGAAGTAATCATGC
VP0117	CTCACACAACACTTTCTG/GCGCGAATTCAGACAATCACACCGATAG
VPA0198	CTCTGGTTCATTGTCTTG/GTCTTGCTGATTCTGATG
VPA1176	TCAGGTACGCTTGCTTCAC/CGTTGCTTGGTAGTGGTAATAG
VP0699	GGAGAATACCTAGCAGAG/AGTATCACGATGTGTCAG
VP2979	TTTCTATCCGTTGGCTAC/CTGACTTACATCGTGGAC
16S rDNA	GACACGGTCCAGACTCCTAC/GGTGCTTCTTCTGTCGCTAAC
DNase I footprinting
scrA	TCGCAGTTGAACAAATATGACG/CCATTAAGTGACTTTAGTTCC
scrG	TAAATACTATTAGGTAACGCG/CAGGGAAATGAAGTAATCATGC
VP0117	CACACTAAAGGTCACAAGCAAG/AGACAATCACACCGATAG
VPA0198	CTTACCTTGGCGTACCTCTTG/ACTGCTTTCTGGGTGAATAACG
VPA1176	GCCATATTCCAAACTCGTTGTG/TGCGTAAGCCAAGTTGATGAG
VP0699	CCACTCCTCTTACAAGAATGAG/AATATCGGATTCCAAGATTC
VP2979	AGCAGTTCCTCGATTTACCTG/GCTTTTCTAGGCACAAAATGAC
5′-RACE
VPA0198	GCTGATGGCGATGAATGAACACTG/AAAGCGAAAAAGAACAGTGC
	GAACACTGCGTTTGCTGGCTTTGATG/ACTGCTTTCTGGGTGAATAACG