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. 2021 May 19;24(6):102554. doi: 10.1016/j.isci.2021.102554

Figure 3.

Figure 3

Neutralization of GDF15 does not modulate weight loss and anorexia induced by a low dose of LPS

(A) GDF15 and (B) KCGRO plasma levels in mice after intraperitoneal injection of a 0.1 mg/kg LPS dose. (C) Schematic representation of the 2 study designs. Changes in (D) body weight and (E) cumulative food intake in fed mice after a single dose of LPS (0.1 mg/kg) and treatment with either GDF15-neutralizing mAB2 or control IgG. Changes in (F) body weight and (G) cumulative food intake in fasted mice after single dose of LPS (0.1 mg/kg) and treatment with either GDF15 neutralizing mAB2 or control IgG. ∗∗∗∗ p value < 0.0001 vs. LPS + IgG or LPS + mAB2; ^ p value < 0.05 vs. LPS + IgG. Food intake (fasted mice) in the LPS + mAB2 group tended to be higher than the LPS + IgG (p = 0.052). Data in (D) were analyzed using Welch's heteroscedastic F test. Data in (E, F, G) were analyzed using ANOVA with Tukey’s multiple comparison adjustment. Data represented as mean ± SEM. N = 10 per group.

BW, body weight; FW, food weight; GDF15, growth differentiation factor 15; IgG, immunoglobulin G; LPS, lipopolysaccharide; SEM, standard error of the mean.