Figure 6.

GDF15 deficiency does not modulate weight loss, anorexia, or mortality induced with a sublethal dose of LPS

(A) Body weight and (B) cumulative food intake after 5 mg/kg LPS injection in WT and GDF15 KO mice. ∗ p value < 0.05, ∗∗ p value < 0.01, ∗∗∗ p value < 0.001 vs. WT saline; ^ p value < 0.05, ^^ p value < 0.01, ^^^ p value < 0.001 vs. KO saline; # p value < 0.05 vs. KO LPS. Data were analyzed using a longitudinal mixed-effects ANOVA. (C) Health score representing overall condition of WT and GDF15 KO mice after 5 mg/kg LPS dose based on their appearance, natural behavior, provoked behavior, and body condition scores. (D) Kaplan-Meier survival curve indicating % survival in WT and GDF15 KO mice after LPS injection. ∗ p value < 0.05 vs. WT saline. Survival times are statistically significantly different between treatment groups p < 0.014. Failure time data were compared between treatment groups with Kaplan-Meier analysis using the log rank test. Data represented as mean ± SEM. N = 6–12 per group (n = 12 WT saline, n = 12 WT LPS, n = 6 KO saline, n = 12 KO LPS).

GDF15, growth differentiation factor 15; KO, knockout; LPS, lipopolysaccharide; SEM, standard error of the mean; WT, wild-type.