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. 2021 Jun 18;9(6):e002195. doi: 10.1136/jitc-2020-002195

Figure 1.

Figure 1

Intraperitoneal colon cancer cells established a highly angiogenic and immunosuppressive milieu within the peritoneal cavity. Mice were intraperitoneally implanted with MC38 colon cancer cells and temporal changes were serially analyzed. (A) Schematic diagram depicting the experimental schedule. (B) Representative images and comparisons of peritoneal tumors. (C) Representative images and comparisons of the peritoneal tumor blood vessels. (D) Representative images and comparisons of malignant ascites over time. (E, F) Representative images and comparisons of CD31+ blood vessels (E), and CD11b+ F4/80+ tumor-associated macrophages (TAMs), CD206+ M2-like macrophages, and iNOS+ M1-like macrophages (F). (G) Representative images and comparisons of CD8+ T cells. Data are pooled from two experiments with n=6 per group (B–G). Values are shown as the mean±SD. *P<0.05; ANOVA with Tukey post hoc test. Scale bar=5 mm (C), 100 µm (E–G). ANOVA, analysis of variance.