FIGURE 8.

Overview of the electrophysiological recordings and neuronal characteristics among the IS, IS + SB269970, IS + AS19 + EA, and IS + EA groups. (A) Spontaneous activity was recorded for 60 s at 30 min postfinding the activated neurons for stabilization among the indicated groups. Spontaneous activity (B), LES-evoked responses (C), and HES-evoked responses (D) of WDR neurons recorded 2 h after the 4th IS in the TCC of none manipulation (IS and IS + SB269970) (E,F) and EA (IS + AS19 + EA and IS + EA) (G,H) rats. When four groups were compared for electrophysiology, a Kruskal-Wallis test was used, and when only two groups were compared, a Mann-Whitney U test was used. Group values are indicated by mean ± SEM. *P < 0.05, **P < 0.01. Tc, the stimulation intensities required to evoke neuronal activity with a conductive velocity of 0.4–2 m s^–1, namely about 2 mA. 5-HT₇R, 5-hydroxytryptamine (5-HT)₇ receptor; LES, low-intensity electrostimulation; HES, high-intensity electrostimulation; WDR, wide-dynamic range; TNC, trigeminal nucleus caudalis; aCSF, artificial cerebrospinal fluid; EA, electroacupuncture; IS, inflammatory soup; AS19, 5-HT7R agonist; SB269970, 5-HT7R antagonist.