Figure 2.

Effects of adrenodoxin on CYP11B2 substrate binding. Changes in absorbance observed upon CYP11B2 titrated with increasing 11-deoxycorticosterone substrate in the absence and presence of increasing amounts of adrenodoxin (A) and for the adrenodoxin–CYP11B2 fusion protein (C). For each experiment a CYP11B2 concentration of 1 μM was used. Data shown are technical triplicates. Analysis using a one-site hyperbolic binding equation yields the fitted dissociation constants and maximum absorbance change at saturation (ΔA_max) in the bottom panel (B).